Answer:

Correct Answer: D

Solution:

• The Southern hybridisation technique, also known as Southern blotting, is a method used to detect specific DNA sequences in DNA samples. The process involves several key steps:

1. Electrophoresis: DNA fragments are separated based on size using gel electrophoresis.
2. Blotting: The separated DNA fragments are transferred from the gel onto a membrane (usually nylon or nitrocellulose).
3. Hybridisation: A labeled DNA probe is used to hybridize to the complementary DNA sequence on the membrane.
4. Detection: The hybridized DNA is then detected, often using autoradiography if the probe is radioactive.

PCR (Polymerase Chain Reaction) is not a part of the Southern hybridisation technique. PCR is a separate technique used to amplify specific DNA sequences.