NEET Solved Paper 2014 Question 36
Question: An analysis of chromosomal DNA using the Southern hybridisation technique does not use [AIPMT 2014]
Options:
A) electrophoresis
B) blotting
C) autoradiography
D) PCR
Show Answer
Answer:
Correct Answer: D
Solution:
- The Southern hybridisation technique, also known as Southern blotting, is a method used to detect specific DNA sequences in DNA samples. The process involves several key steps:
- Electrophoresis: DNA fragments are separated based on size using gel electrophoresis.
- Blotting: The separated DNA fragments are transferred from the gel onto a membrane (usually nylon or nitrocellulose).
- Hybridisation: A labeled DNA probe is used to hybridize to the complementary DNA sequence on the membrane.
- Detection: The hybridized DNA is then detected, often using autoradiography if the probe is radioactive.
PCR (Polymerase Chain Reaction) is not a part of the Southern hybridisation technique. PCR is a separate technique used to amplify specific DNA sequences.
BETA
