Biotechnology Principles And Processes Question 1

Question: Main steps in the formation of recombinant DNA are given below. Arrange these steps in a correct sequence.

A Insertion of recombinant DNA into the host cell.

B. Cutting of DNA at specific location by restriction enzyme.

C. Isolation of desired DNA fragment.

D. Amplification of gene of interest using PCR.

Choose the correct answer from the options given below:

(1) B, D, A, C

(3) C, A, D, B

(2). B, C, D, A

(4), C, B, D, A

Show Answer

Answer: (4), C, B, D, A

Explanation-

Here is the explanation for the sequence:

  1. Isolation of desired DNA fragment (C)
  2. Cutting of DNA at specific location by restriction enzyme (B)
  3. Amplification of gene of interest using PCR (D)
  4. Insertion of recombinant DNA into the host cell (A)


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